The Optimization of Binary Mobile Phase Composition in Multi-Step Linear Gradient Elution

1、

On the basis of the retention mechanism of displacement adsorption multi-interaction in liquid chromatography, a new retention model which can be used in arbitrary multiple binary linear gradient chromatography was established and the relative retention fundamental equation was derived.

根据顶替吸附多种相互作用的液相色谱保留机理，本文提出了一个新的适用于任意多阶二元线性梯度色谱的保留模型并导出了相应的保留值基本方程。

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2、

A C_ 18 column was used with acetonitrile-0.1% acetic acid solution as the mobile phase to determine scutellarein, luteolin and apigenin, and a linear gradient elution from 15% to 60% acetonitrile in 40 min was applied.

测定野黄芩素、木犀草素和芹菜素以乙腈-0.1%醋酸水溶液为流动相，梯度洗脱（0→40min，乙腈15%→60%）。

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3、

Carotenoids were separated by linear gradient elution using ethyl acetate and water-acetonitrile ( 1:9, V/ V) as mobile phase.

类胡萝卜素亦采用线性梯度洗脱，以乙酸乙酯作为A相，B相为乙腈-水（体积比9∶1）。保留时间、相对保留时间、光谱法、加标定性，内标法定量。

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4、

Objective Two HPLC methods with linear gradient elution for determination of the related substances of amoxicillin-potassium clavulanate were established and compared.

目的建立两种HPLC线性梯度洗脱测定阿莫西林/克拉维酸钾有关物质的方法，并对其进行比较。

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5、

Objective: To develop a HPLC linear gradient elution method for rapid separation of the components of bleomycin, and for determination of bleomycin A2 and bleomycin B2. Method;

目的：研究应用反相离子对色谱梯度洗脱法以较快速度分离硫酸博莱霉素组分，并尝试梯度洗脱条件下测定其有效成分A2、B2的含量。

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6、

Select both shapes and create the same colored Linear Gradient as the Text.

选择这两个形状和创建相同的彩色线性梯度作为文本.

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7、

Because of the printing process is non-linear gradient, in prepress compensation should be done.

由于印刷工艺阶调是非线性传递, 在印前应做补偿调整.

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8、

Now select the Linear Gradient Tool.

现在选择线形渐变工具.

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9、

So, in a sense, we'd not really be offering "free college," but simply an extension of the overworked and under-resourced free public education system.

所以，某种意义上，我们不是要提供“免费大学教育”，而只是提供了对现在过劳和资源紧张的免费公立教育一个延伸。译文属可可英语原创，仅供学习和交流使用，未经许可，请勿转载。

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10、

He said that the company phased out long-chain PFCs at the beginning of this year, requiring that all materials meet the standards laid out in Nike's Restricted Substances List.

他说，在今年年初公司淘汰长链氟化合物，要求所有材质均符合耐克的受限物质清单的标准。译文属可可英语原创，仅供学习和交流使用，未经允许不得转载。

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11、

And remember to join us on wednesday afternoon at 5:30 and Tuesday morning at 9:30 for the latest developments in Science and Technology and Sci-Tech today.

欢迎每周三下午5:30或周二早上9:30收看Sci-tech的最新科技动态。注：本篇文章英文来自普特论坛，译文属可可英语原创，未经允许，请勿转载。

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12、

What we are dealing with here is "signalling"-in this case, how we communicate the message that work's getting done, which doesn't always mean work's really getting done.

我们在这里讨论的是“信号”，是指在这种情况下，我们如何传达正在工作这种信息，而这并不总是意味着真正在工作。译文属可可英语原创，仅供学习与交流，未经允许，不得转载。

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13、

The auction will take place on www.elle.com.

网上竞拍将会在www.elle.com网上进行。注：本篇文章英文来自普特论坛，译文属可可英语原创，未经允许，请勿转载。

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14、

Tell me about it in the comments below or share your own photo on social media using the hashtag\# FindingAsia!

可以在下面的评论中告诉我，或者在社交媒体上用标签#FindingAsia分享你自己的照片。译文属可可英语原创，仅供学习和交流使用，未经许可，请勿转载。

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15、

From hottest gadgets, to the latest tech news, we stream alive, 5 days a week, at Cnet.com/live.

不管是最热的电子配件，还是最新的科技新闻，我们都将及时为您报道，一周5期，精彩尽在Cnet.com。注：本篇文章英文来自普特论坛，译文属可可英语原创，未经允许，请勿转载。

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16、

The event brought together about 14 hundred red heads from 52 countries.

来自52个国家的将近1千4百名红发者参加了这场盛会。注：译文属可可英语原创，未经允许，请勿转载。

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17、

David Shukman, BBC news, in Leeds

David Shukman，BBC新闻，利兹市报道。注：本篇文章英文来自普特论坛，译文属可可英语原创，未经允许，请勿转载。

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18、

Readers, are you one of these high-energy, no-sleep jugglers? Can some people actually function this way indefinitely, leaving the rest of us lagging behind the evolutionary curve?

各位读者，你也是这种精力充沛的不睡觉超人吗？这种人是否能够永远这么几乎不停工地运转，将我们这些凡夫俗子在进化进程中远远甩在身后？（文字来源：可可英语）

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19、

Steve Coleman, the Associated Press with AP News Minute.

史蒂夫·科勒曼报道，这里是美联社新闻一分钟。注：译文属可可英语原创，未经允许，请勿转载。

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20、

In 2014, the Baby singer was baptised by Hillsong Church Pastor Carl Lentz 。

在2014年，他在希尔送教堂牧师卡尔·伦茨的主持下接受了洗礼。

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