aif

RESULTS: The 1.5 kb AIF gene was successfully isolated , and cloned into pET 32 a ( + ) vector.

结果: 获得了去掉线粒体定位信号的AIF基因, 并克隆到pET32a ( + ) 载体中,经酶切与测序鉴定完全正确.

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RESULTS: The eukaryotic expression vector containing the truncated human AIF ( AIF 1-400 ) gene was successfully constructed.

结果: 经酶切鉴定与测序证实,带有 AIF1 -400的真核表达载体构建成功.

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RESULTS: The eukaryotic expression vector pIRES 2-EGFP containing the truncated human AIF ( AIF � � 1-480 ) gene was successfully constructed.

结果: 成功构建了人截短型AIF ( AIF△1480 ) 基因的真核表达载体.

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Furthermore , AIF expression was up-regulated.

凋亡诱导因子 AIF 蛋白的表达也有所增加.

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Apoptosis-inducing factor ( AIF ) is a mi-tochondrial intermembrane space flavoproteln.

凋亡诱导因子 ( apoptosis-inducingfactor, AIF ) 是位于线粒体膜间隙的一种黄素蛋白.

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After renaturation, AIF protein binded DNA and induced nuclear apoptosis.

经EMSA与 Hoechst33258 染色实验证实:获得的AIF蛋白具有良好地与DNA结合并可诱导肿瘤细胞核凋亡的能力,凋亡率为37%.

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RESULTS: The eukaryotic expression vector pIRES 2-EGFP containing the truncated human AIF ( AIF 1-480 ) gene was successfully constructed.

结果: 成功构建了人截短型AIF ( AIF1480 ) 基因的真核表达载体.

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AIF-1 is originally cloned from rat heart allograft.

AIF -1 最初发现于同种大鼠的异体移植心脏.

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AIf I can't get tickets, I'll watch it on television.

如果我买不到票, 我就在电视上看比赛.

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AIF could be phosphorylated by c-Abl.

AIF可以被c-Abl 磷酸化,且c-Abl能 提高AIF的表达量.

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Jinguzul Aya'aif 0 Jingizu! came a wailing cry.

传来了一阵悲伤的叫声:悲伤! 哦,悲伤!

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AIF _ 3— Al _ 2 O _ 3 ( sat. ) melts at 1000℃ by a steady state potentiostatic method.

用稳态恒电位法及脉冲技术,对1000℃下2.7NaF·AlF_3-Al_2O_3 ( 饱和 ) 熔体中SnO_ 2基情性阳极上氧析出的超电压作了测定.

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RESULTS: The eukaryotic expression vector containing the truncated human AIF ( AIF � � 1-400 ) gene was successfully constructed.

结果: 经酶切鉴定与测序证实,带有 AIF △ 1-400 的真核表达载体构建成功.

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